Life Sciences Institute

Research Output 1969 2018

2018

Autophagy and inflammation: A special review issue

Deretic, V. & Klionsky, D. J. Jan 1 2018 (Accepted/In press) In : Autophagy. p. 1-2 2 p.

Research output: Contribution to journalArticle

Autophagy
Inflammation
Quality Control
Immunity
Obesity

Big data in cryoEM: automated collection, processing and accessibility of EM data

Baldwin, P. R., Tan, Y. Z., Eng, E. T., Rice, W. J., Noble, A. J., Negro, C. J., Cianfrocco, M. A., Potter, C. S. & Carragher, B. Jun 1 2018 In : Current Opinion in Microbiology. 43, p. 1-8 8 p.

Research output: Contribution to journalReview article

Electron Microscopy
Explosions
Automation
Motion Pictures
Cloud Computing

Biocatalytic site- and enantioselective oxidative dearomatization of phenols

Baker Dockrey, S. A., Lukowski, A. L., Becker, M. R. & Narayan, A. R. H. Feb 1 2018 In : Nature Chemistry. 10, 2, p. 119-125 7 p.

Research output: Contribution to journalArticle

Phenols
Catalyst selectivity
Catalysts
Enzymes
Biological Products
1 Citations

Cargo recognition and degradation by selective autophagy

Gatica, D., Lahiri, V. & Klionsky, D. J. Mar 1 2018 In : Nature Cell Biology. 20, 3, p. 233-242 10 p.

Research output: Contribution to journalReview article

Autophagy
Organelles
Ligands
Food
Proteins

Characterization of MC4R regulation of the Kir7.1 channel using the Tl+ flux assay

Litt, M. J., Cone, R. D. & Ghamari-Langroudi, M. Jan 1 2018 Methods in Molecular Biology. Humana Press Inc., Vol. 1684, p. 211-222 12 p. (Methods in Molecular Biology; vol. 1684)

Research output: Chapter in Book/Report/Conference proceedingChapter

Receptor, Melanocortin, Type 4
Potassium Channels
Patch-Clamp Techniques
Drug Discovery
Electrolytes

Chemistry of a Unique Polyketide-like Synthase

Chun, S. W., Hinze, M. E., Skiba, M. A. & Narayan, A. R. H. Feb 21 2018 In : Journal of the American Chemical Society. 140, 7, p. 2430-2433 4 p.

Research output: Contribution to journalArticle

Saxitoxin
Polyketide Synthases
Polyketides
Enzymes
Carbon

Design of the First-in-Class, Highly Potent Irreversible Inhibitor Targeting the Menin-MLL Protein–Protein Interaction

Xu, S., Aguilar, A., Xu, T., Zheng, K., Huang, L., Stuckey, J., Chinnaswamy, K., Bernard, D., Fernández-Salas, E., Liu, L., Wang, M., McEachern, D., Przybranowski, S., Foster, C. & Wang, S. Feb 5 2018 In : Angewandte Chemie - International Edition. 57, 6, p. 1601-1605 5 p.

Research output: Contribution to journalArticle

Gene expression
Mass spectrometry
Genes
Crystal structure
Proteins
1 Citations

Heat shock protein 70 (Hsp70) suppresses RIP1-dependent apoptotic and necroptotic cascades

Srinivasan, S. R., Cesa, L. C., Li, X., Julien, O., Zhuang, M., Shao, H., Chung, J., Maillard, I., Wells, J. A., Duckett, C. S. & Gestwicki, J. E. Jan 1 2018 In : Molecular Cancer Research. 16, 1, p. 58-68 11 p.

Research output: Contribution to journalArticle

HSP70 Heat-Shock Proteins
Cell Death
Apoptosis
Molecular Chaperones
Proteolysis

High throughput protease profiling comprehensively defines active site specificity for thrombin and ADAMTS13

Kretz, C. A., Tomberg, K., Van Esbroeck, A., Yee, A. & Ginsburg, D. Dec 1 2018 In : Scientific Reports. 8, 1, 2788

Research output: Contribution to journalArticle

Thrombin
Catalytic Domain
Peptide Hydrolases
Peptides
Bacteriophages

Human antibody recognition of antigenic site IV on Pneumovirus fusion proteins

Mousa, J. J., Binshtein, E., Human, S., Fong, R. H., Alvarado, G., Doranz, B. J., Moore, M. L., Ohi, M. D. & Crowe, J. E. Feb 1 2018 In : PLoS pathogens. 14, 2, e1006837

Research output: Contribution to journalArticle

Pneumovirus
Respiratory Syncytial Viruses
Metapneumovirus
Antibodies
Monoclonal Antibodies

Lysophosphatidic acid regulates the motility of MCF10CA1a breast cancer cell sheets via two opposing signaling pathways

Stuelten, C. H., Lee, R. M., Losert, W. & Parent, C. A. May 1 2018 In : Cellular Signalling. 45, p. 1-11 11 p.

Research output: Contribution to journalArticle

Cell Movement
Breast Neoplasms
Time-Lapse Imaging
Actomyosin
Cadherins
1 Citations

Macropinocytosis, mTORC1 and cellular growth control

Yoshida, S., Pacitto, R., Inoki, K. & Swanson, J. Apr 1 2018 In : Cellular and Molecular Life Sciences. 75, 7, p. 1227-1239 13 p.

Research output: Contribution to journalReview article

Growth
Intercellular Signaling Peptides and Proteins
Monomeric GTP-Binding Proteins
Growth Factor Receptors
Lysosomes

Microinjection of CRISPR/cas9 protein into channel catfish, Ictalurus punctatus, embryos for gene editing

Elaswad, A., Khalil, K., Cline, D., Page-McCaw, P., Chen, W., Michel, M., Cone, R. & Dunham, R. Jan 20 2018 In : Journal of Visualized Experiments. 2018, 131, e56275

Research output: Contribution to journalArticle

Clustered Regularly Interspaced Short Palindromic Repeats
Ictaluridae
CRISPR-Associated Proteins
Microinjections
Embryonic Structures

New Insights into Graft-Versus-Host Disease and Graft Rejection

Perkey, E. & Maillard, I. Jan 24 2018 In : Annual Review of Pathology: Mechanisms of Disease. 13, p. 219-245 27 p.

Research output: Contribution to journalArticle

Graft Rejection
Graft vs Host Disease
T-Lymphocytes
Isoantigens
Microbiota

Pharmacologic evidence for a putative conserved allosteric site on opioid receptors

Livingston, K. E., Stanczyk, M. A., Burford, N. T., Alt, A., Canals, M. & Traynor, J. R. Feb 1 2018 In : Molecular Pharmacology. 93, 2, p. 157-167 11 p.

Research output: Contribution to journalArticle

Allosteric Site
mu Opioid Receptor
Opioid Receptors
delta Opioid Receptor
kappa Opioid Receptor

Regulation of autophagic lysosome reformation by kinesin 1, clathrin and phosphatidylinositol-4,5-bisphosphate

Liu, X. & Klionsky, D. J. Jan 2 2018 In : Autophagy. 14, 1, p. 1-2 2 p.

Research output: Contribution to journalEditorial

Kinesin
Clathrin
Autophagy
Phosphatidylinositols
Lysosomes
1 Citations

Secretory autophagy holds the key to lysozyme secretion during bacterial infection of the intestine

Delorme-Axford, E. & Klionsky, D. J. Jan 1 2018 (Accepted/In press) In : Autophagy. p. 1-3 3 p.

Research output: Contribution to journalArticle

Autophagy
Muramidase
Bacterial Infections
Intestines
Paneth Cells

Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters

Grunseich, C., Wang, I. X., Watts, J. A., Burdick, J. T., Guber, R. D., Zhu, Z., Bruzel, A., Lanman, T., Chen, K., Schindler, A. B., Edwards, N., Ray-Chaudhury, A., Yao, J., Lehky, T., Piszczek, G., Crain, B., Fischbeck, K. H. & Cheung, V. G. Feb 1 2018 In : Molecular Cell. 69, 3, p. 426-437.e7

Research output: Contribution to journalArticle

Amyotrophic Lateral Sclerosis
DNA Methylation
Transforming Growth Factor beta
Nucleic Acids
Mutation

Subcellular specialization and organelle behavior in germ cells

Yamashita, Y. M. Jan 1 2018 In : Genetics. 208, 1, p. 19-51 33 p.

Research output: Contribution to journalReview article

Germ Cells
Organelles
Gametogenesis
Centrosome
Endoplasmic Reticulum

The SWI/SNF chromatin-remodeling factors BAF60a, b, and c in nutrient signaling and metabolic control

Wang, R. R., Pan, R., Zhang, W., Fu, J., Lin, J. D. & Meng, Z. X. Feb 1 2018 In : Protein and Cell. 9, 2, p. 207-215 9 p.

Research output: Contribution to journalReview article

Chromatin Assembly and Disassembly
Nutrients
Chromatin
Food
Physiology

Thiosulfate sulfurtransferase-like domain-containing 1 protein interacts with thioredoxin

Libiad, M., Motl, N., Akey, D. L., Sakamoto, N., Fearon, E. R., Smith, J. L. & Banerjee, R. Jan 1 2018 In : Journal of Biological Chemistry. 293, 8, p. 2675-2686 12 p.

Research output: Contribution to journalArticle

Thiosulfate Sulfurtransferase
Thioredoxins
Sulfides
Sulfur
Thiosulfates

Uncoupling exercise bioenergetics from systemic metabolic homeostasis by conditional inactivation of Baf60 in skeletal muscle

Meng, Z. X., Tao, W., Sun, J., Wang, Q., Mi, L. & Lin, J. D. Jan 1 2018 In : Diabetes. 67, 1, p. 85-97 13 p.

Research output: Contribution to journalArticle

Energy Metabolism
Skeletal Muscle
Homeostasis
Muscles
Chromatin Assembly and Disassembly

Vacuole-mediated selective regulation of TORC1-Sch9 signaling following oxidative stress

Takeda, E., Jin, N., Itakura, E., Kira, S., Kamada, Y., Weisman, L. S., Noda, T. & Matsuura, A. Feb 15 2018 In : Molecular Biology of the Cell. 29, 4, p. 510-522 13 p.

Research output: Contribution to journalArticle

Vacuoles
Oxidative Stress
Nitrogen
Saccharomycetales
Membranes
1 Citations

X-linked inhibitor of apoptosis protein (XIAP) is a client of heat shock protein 70 (Hsp70) and a biomarker of its inhibition

Cesa, L. C., Shao, H., Srinivasan, S. R., Tse, E., Jain, C., Zuiderweg, E. R. P., Southworth, D. R., Mapp, A. K. & Gestwicki, J. E. Jan 1 2018 In : Journal of Biological Chemistry. 293, 7, p. 2370-2380 11 p.

Research output: Contribution to journalReview article

X-Linked Inhibitor of Apoptosis Protein
HSP70 Heat-Shock Proteins
Biomarkers
Cells
Tumors
2017

A B Cell Regulome Links Notch to Downstream Oncogenic Pathways in Small B Cell Lymphomas

Ryan, R. J. H., Petrovic, J., Rausch, D. M., Zhou, Y., Lareau, C. A., Kluk, M. J., Christie, A. L., Lee, W. Y., Tarjan, D. R., Guo, B., Donohue, L. K. H., Gillespie, S. M., Nardi, V., Hochberg, E. P., Blacklow, S. C., Weinstock, D. M., Faryabi, R. B., Bernstein, B. E., Aster, J. C. & Pear, W. S. Oct 17 2017 In : Cell Reports. 21, 3, p. 784-797 14 p.

Research output: Contribution to journalArticle

B-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Mantle-Cell Lymphoma
B-Lymphocytes
Cells
1 Citations

A Bifunctional Amino Acid Enables Both Covalent Chemical Capture and Isolation of in Vivo Protein–Protein Interactions

Joiner, C. M., Breen, M. E., Clayton, J. & Mapp, A. K. Jan 17 2017 In : ChemBioChem. 18, 2, p. 181-184 4 p.

Research output: Contribution to journalArticle

Amino Acids
Alkynes
Proteins
Crosslinking
Azides
1 Citations

A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation

Wu, S. J., Niknafs, Y. S., Kim, S. H., Oravecz-Wilson, K., Zajac, C., Toubai, T., Sun, Y., Prasad, J., Peltier, D., Fujiwara, H., Hedig, I., Mathewson, N. D., Khoriaty, R., Ginsburg, D. & Reddy, P. Jun 27 2017 In : Cell Reports. 19, 13, p. 2645-2656 12 p.

Research output: Contribution to journalArticle

Cross-Priming
SNARE Proteins
Antigen Presentation
Dendritic Cells
Antigens

A few words of introduction from your new diabetes editorial board

Myers, M. G., D'Alessio, D. A., Gannon, M. A., Seeley, R. J., Burant, C. F., Doria, A., Grant, M. B., Herman, W. H., Herold, K. C., Horowitz, J. F., Lin, J. D., Lumeng, C. N. K., MacDougald, O. A., Pietropaolo, M., Pop-Busui, R., Satin, L., Stoffers, D. A. & White, M. F. Jan 1 2017 In : Diabetes. 66, 1, p. 3-4 2 p.

Research output: Contribution to journalComment/debate

A genome-wide association study of anorexia nervosa suggests a risk locus implicated in dysregulated leptin signaling

Li, D. , Chang, X. , Connolly, J. J. , Tian, L. , Liu, Y. , Bhoj, E. J. , Robinson, N. , Abrams, D. , Li, Y. R. , Bradfield, J. P. , Kim, C. E. , Li, J. , Wang, F. , Snyder, J. , Lemma, M. , Hou, C. , Wei, Z. , Guo, Y. , Qiu, H. , Mentch, F. D. & 30 others Thomas, K. A., Chiavacci, R. M., Cone, R., Li, B., Sleiman, P. A., Hakonarson, H., Perica, V. B., Franklin, C. S., Floyd, J. A. B., Thornton, L. M., Huckins, L. M., Southam, L., Rayner, N. W., Tachmazidou, I., Schmidt, U., Tozzi, F., Kiezebrink, K., Hebebrand, J., Gorwood, P., Adan, R. A. H., Kas, M. J. H., Favaro, A., Santonastaso, P., Fernánde-Aranda, F., Gratacos, M., Rybakowski, F., Dmitrzak-Weglarz, M., Kaprio, J., Keski-Rahkonen, A. & Raevuori-Helkamaa, A. Dec 1 2017 In : Scientific Reports. 7, 1, 3847

Research output: Contribution to journalArticle

Genome-Wide Association Study
Anorexia Nervosa
Leptin
Phenotype
Single Nucleotide Polymorphism
1 Citations

Aggregation of Mod5 is affected by tRNA binding with implications for tRNA gene-mediated silencing

Read, D. F., Waller, T. J., Tse, E., Southworth, D. R., Engelke, D. R. & Smaldino, P. J. Jun 1 2017 In : FEBS Letters. 591, 11, p. 1601-1610 10 p.

Research output: Contribution to journalLetter

Gene Silencing
Transfer RNA
Agglomeration
Genes
RNA
3 Citations

Altered channel conductance states and gating of GABAA receptors by a pore mutation linked to Dravet syndrome

Hernandez, C. C., Kong, W., Hu, N., Zhang, Y., Shen, W., Jackson, L., Liu, X., Jiang, Y. & Macdonald, R. L. Jan 1 2017 In : eNeuro. 4, 1, e0251-16.2017

Research output: Contribution to journalArticle

Myoclonic Epilepsy
gamma-Aminobutyric Acid
GABA-A Receptors
Mutation
Missense Mutation
2 Citations

A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens

Kinnel, R. B., Esquenazi, E., Leao, T., Moss, N., Mevers, E., Pereira, A. R., Monroe, E. A., Korobeynikov, A., Murray, T. F., Sherman, D., Gerwick, L., Dorrestein, P. C. & Gerwick, W. H. May 26 2017 In : Journal of Natural Products. 80, 5, p. 1514-1521 8 p.

Research output: Contribution to journalArticle

Cyanobacteria
Biological Products
Genes
Biosynthesis
Amidinotransferases
1 Citations

A Mononuclear Iron-Dependent Methyltransferase Catalyzes Initial Steps in Assembly of the Apratoxin A Polyketide Starter Unit

Skiba, M. A., Sikkema, A. P., Moss, N. A., Tran, C. L., Sturgis, R. M., Gerwick, L., Gerwick, W. H., Sherman, D. H. & Smith, J. L. Dec 15 2017 In : ACS Chemical Biology. 12, 12, p. 3039-3048 10 p.

Research output: Contribution to journalArticle

Bacterial Gene Expression Regulation
Depsipeptides
Polyketides
Protein Conformation
Decarboxylation
2 Citations

An aberrant NOTCH2-BCR signaling axis in B cells from patients with chronic GVHD

Poe, J. C. , Jia, W. , Su, H. , Anand, S. , Rose, J. J. , Tata, P. V. , Suthers, A. N. , Jones, C. D. , Kuan, P. F. , Vincent, B. G. , Serody, J. S. , Horwitz, M. E. , Ho, V. T. , Pavletic, S. Z. , Hakim, F. T. , Owzar, K. , Zhang, D. , Blazar, B. R. , Siebel, C. W. , Chao, N. J. & 2 others Maillard, I. & Sarantopoulos, S. Nov 9 2017 In : Blood. 130, 19, p. 2131-2145 15 p.

Research output: Contribution to journalArticle

B-Lymphocytes
Cells
Grafts
Chemical activation
Graft vs Host Disease
2 Citations

An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and Rapid Restriction of Developmental Potential during Asymmetric Stem Cell Division

Janssens, D. H., Hamm, D. C., Anhezini, L., Xiao, Q., Siller, K. H., Siegrist, S. E., Harrison, M. M. & Lee, C. Y. Feb 27 2017 In : Developmental Cell. 40, 4, p. 367-380.e7

Research output: Contribution to journalArticle

Ear Protective Devices
Asymmetric Cell Division
Histones
Histone Deacetylases
Neural Stem Cells
2 Citations

An intramolecular interaction within the lipid kinase Fab1 regulates cellular phosphatidylinositol 3,5-bisphosphate lipid levels

Lang, M. J., Strunk, B. S., Azad, N., Petersen, J. L. & Weisman, L. S. Apr 1 2017 In : Molecular Biology of the Cell. 28, 7, p. 858-864 7 p.

Research output: Contribution to journalArticle

Cysteine
Phosphotransferases
Lipids
Phosphatidylinositols
Mutation
1 Citations

A novel role for a glycolytic pathway kinase in regulating autophagy has implications in cancer therapy

Ariosa, A. R. & Klionsky, D. J. Jul 3 2017 In : Autophagy. 13, 7, p. 1091-1092 2 p.

Research output: Contribution to journalEditorial

Autophagy
Phosphotransferases
Neoplasms
Therapeutics
Class III Phosphatidylinositol 3-Kinases
1 Citations

A pathway of targeted autophagy is induced by DNA damage in budding yeast

Eapen, V. V., Waterman, D. P., Bernard, A., Schiffmann, N., Sayas, E., Kamber, R., Lemos, B., Memisoglu, G., Ang, J., Mazella, A., Chuartzman, S. G., Loewith, R. J., Schuldiner, M., Denic, V., Klionsky, D. J. & Haber, J. E. Feb 14 2017 In : Proceedings of the National Academy of Sciences of the United States of America. 114, 7, p. E1158-E1167

Research output: Contribution to journalArticle

Saccharomycetales
Autophagy
DNA Damage
Mutagens
Checkpoint Kinase 2
2 Citations

APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA

Bohn, J. A., Thummar, K., York, A., Raymond, A., Brown, W. C., Bieniasz, P. D., Hatziioannou, T. & Smith, J. L. Dec 1 2017 In : Nature Communications. 8, 1, 1021

Research output: Contribution to journalArticle

RNA
Cytidine Deaminase
primates
proteins
swine
2 Citations

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation

Zhou, H., Lu, J., Liu, L., Bernard, D., Yang, C. Y., Fernandez-Salas, E., Chinnaswamy, K., Layton, S., Stuckey, J., Yu, Q., Zhou, W., Pan, Z., Sun, Y. & Wang, S. Dec 1 2017 In : Nature Communications. 8, 1, 1150

Research output: Contribution to journalArticle

Cullin Proteins
inhibitors
proteins
Molecules
molecules
1 Citations

A Single Active Site Mutation in the Pikromycin Thioesterase Generates a More Effective Macrocyclization Catalyst

Koch, A. A., Hansen, D. A., Shende, V. V., Furan, L. R., Houk, K. N., Jiménez-Osés, G. & Sherman, D. H. 2017 In : Journal of the American Chemical Society. 139, 38, p. 13456-13465 10 p.

Research output: Contribution to journalArticle

Catalytic Domain
Reaction kinetics
Mutation
Catalysts
Substrates
1 Citations

A snapshot of cryo-EM

Skiniotis, G. Jan 1 2017 In : Protein Science. 26, 1, p. 5-7 3 p.

Research output: Contribution to journalEditorial

4 Citations

Augmenting brain metabolism to increase macro- and chaperone-mediated autophagy for decreasing neuronal proteotoxicity and aging

Loos, B., Klionsky, D. J. & Wong, E. Sep 1 2017 In : Progress in Neurobiology. 156, p. 90-106 17 p.

Research output: Contribution to journalReview article

Autophagy
Brain
Ketone Bodies
Caloric Restriction
Lysosomes
1 Citations

Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase

Sasaki, T., Lian, S., Khan, A., Llop, J. R., Samuelson, A. V., Chen, W., Klionsky, D. J. & Kishi, S. Feb 1 2017 In : Autophagy. 13, 2, p. 386-403 18 p.

Research output: Contribution to journalArticle

Adenosine Triphosphatases
Autophagy
Drosophila
Clustered Regularly Interspaced Short Palindromic Repeats
Premature Aging

Autophagic membrane delivery through ATG9

Feng, Y. & Klionsky, D. J. Feb 1 2017 In : Cell Research. 27, 2, p. 161-162 2 p.

Research output: Contribution to journalArticle

Autophagy
Membranes
trans-Golgi Network
Starvation
Phosphotransferases
1 Citations

Autophagy regulates DNA repair through SQSTM1/p62

Feng, Y. & Klionsky, D. J. Jun 3 2017 In : Autophagy. 13, 6, p. 995-996 2 p.

Research output: Contribution to journalEditorial

Autophagy
DNA Repair
Ubiquitination
Ubiquitin-Protein Ligases
Double-Stranded DNA Breaks
9 Citations
Autophagy
Saccharomyces cerevisiae
Biomedical Research
Medicine
Nobel Prize
11 Citations

BAG3 Is a Modular, Scaffolding Protein that physically Links Heat Shock Protein 70 (Hsp70) to the Small Heat Shock Proteins

Rauch, J. N., Tse, E., Freilich, R., Mok, S. A., Makley, L. N., Southworth, D. R. & Gestwicki, J. E. Jan 6 2017 In : Journal of Molecular Biology. 429, 1, p. 128-141 14 p.

Research output: Contribution to journalArticle

Small Heat-Shock Proteins
HSP70 Heat-Shock Proteins
Crystallins
Adenosine Triphosphate
Protein Unfolding
2 Citations

BCL6 antagonizes NOTCH2 to maintain survival of human follicular lymphoma cells

Valls, E. , Lobry, C. , Geng, H. , Wang, L. , Cardenas, M. , Rivas, M. , Cerchietti, L. , Oh, P. , Yang, S. N. , Oswald, E. , Graham, C. W. , Jiang, Y. , Hatzi, K. , Agirre, X. , Perkey, E. , Li, Z. , Tam, W. , Bhatt, K. , Leonard, J. P. , Zweidler-McKay, P. A. & 5 others Maillard, I., Elemento, O., Ci, W., Aifantis, I. & Melnick, A. May 1 2017 In : Cancer Discovery. 7, 5, p. 506-521 16 p.

Research output: Contribution to journalArticle

Follicular Lymphoma
Survival
Germinal Center
Genes
Gene Silencing

Blame it on Southern, but it's a western blot

Klionsky, D. J. Jan 2 2017 In : Autophagy. 13, 1, p. 1-2 2 p.

Research output: Contribution to journalEditorial

Southern Blotting
Western Blotting
Molecular Biology
Genome
Polymerase Chain Reaction